Dr. Mamata Mishra*
Objective: Skin wound healing involves the interaction of epidermal and dermal cells, growth factors, and cytokines. Keratinocyte is the most dominant cell type in the epidermis and plays multiple roles for skin repair function. Cultured human primary keratinocytes are required for the treatment of burns, cutaneous wounds diabetic foot ulcers, and cosmetic therapy
Methods: Human primary keratinocytes from different age groups were isolated, characterized and efficacy was analyzed. Primary human keratinocytes were purchased from commercially available sources and their trans-differentiation properties were studied.
Results: Serum-free media with high calcium was used to isolate keratinocytes from different donors with varied age groups. The number of keratinocytes isolated from the total population of cells varied depending upon the age. Periodical photographs were being taken for analysis. Quantitative analysis of percentage expression of CK5 and CK10 has been done in keratinocytes and fibronectin and collagen staining has been done in transdifferentiated fibroblast.
Conclusion: Isolation of primary keratinocytes brings the hope that healthy cultured keratinocytes are useful for the wound microenvironment. Trans-differentiation of keratinocytes to fibroblast provides a new angle for understanding the interaction between keratinocytes and fibroblast in cosmetic surgery and wound healing.
KeywordsSkin wound healing; Trans-differentiation; Cultured keratinocytes; Primary human keratinocytes; Dermal fibroblast
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