Archivos de Microbiología Clínica

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Abstracto

Identification of gyrA Gene in Ciprofloxacin- Resistant Enterococcus Faecalis in Strains Isolated from Clinical Specimens in Hospitals and Clinics of Tabriz and Marand Cities

Jafari-Sales A, Sayyahi J, Akbari-Layeg F, Mizabi-Asl M, Rasi-Bonab F, Abdoli-senejani M, Ezdiyadi M

Background and objective: Resistance to ciprofloxacin in Enterococcus faecalis isolates, especially in urinary tract infections, has caused therapeutic problems. Therefore, the objective of this study molecular identification and isolation of gyrA gene in ciprofloxacin-resistant Enterococcus faecalis in strains isolated from clinical specimens.

Methods: In the current research, 196 enterococci samples prepared from clinical specimens including blood and urine were investigated in hospitals and health centers of Tabriz and Marand cities. Genus and species, and gene of resistance to ciprofloxacin were identified by PCR method and antibiotic sensitivity test. In addition, MIC was identified by E-test.

Results: Among 194 (98.97%) enterococci strains identified, 93 (47.93%) strains belonged to faecalis species. Among 40 specimens resistant to ciprofloxacin, 92.5% of them included gyrA gene.

Conclusion: Ciprofloxacin-resistant Enterococcus faecalis strains have diverse clonality. It means that they are not distributed uniformly and transference of resistance genes in the enterococci population is high. People colonized with these strains are in fact considered stores for releasing of resistance genes in the society, requiring more implementation of caring programs.

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